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![Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. pSample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NOX1-Antibody-Immunohistochemistry-Paraffin-NBP1-31546-img0005.jpg "Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. pSample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: NOX1 Antibody [NBP1-31546] - Blueberry polyphenol extract (BPE) did not affect the expression of NADPH oxidases (NOX) in angiotensin (Ang) II-treated human aortic endothelial cells (HAECs). HAECs were treated with 200 µg/mL of BPE for 1 h then treated with 200 nM of Ang II for 12 h. Protein expression of NOX1 (A,B), NOX2 (C,D), NOX4 (E,F), & NOX5 (G,H) were determined by Western blot. Quantification was performed using Image Lab (Bio-Rad Laboratories, Inc.). Data are expressed as mean ± SD from three (NOX1 & NOX4) & five (NOX2 & NOX5) independent experiments. Values that do not share the same letter are significantly different from each other (p ≤ 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35453301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31546_rabbit-polyclonal-nox1-antibody-31020241517138.jpg)
![Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Western-Blot-NB100-168-img0002.jpg "Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunocytochemistry-Immunofluorescence-NB100-168-img0004.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 23.2) [NB100-168] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:2000 conc. of MGMT antibody (clone MT 23.2) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of MGMT was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunohistochemistry-Paraffin-NB100-168-img0013.jpg)
![Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on HeLa cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Cytometry-NB100-168-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MGMT (MT 23.2) NB100-168 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Immunocytochemistry-Immunofluorescence-NB100-168-img0006.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0008.jpg)
![Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in Human Jurkat Cell Line by Flow Cytometry. Human Jurkat cell line was stained with Mouse Anti- MGMT Monoclonal Antibody (Catalog # NB100-168, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Cytometry-NB100-168-img0011.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Raji cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control NBP2-27231AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0012.jpg)
![Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Flow-Intracellular-NB100-168-img0014.jpg)
![Simple Western: MGMT Antibody (MT 23.2) [NB100-168] - Simple Western lane view shows a specific band for MGMT in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-23-2-Simple-Western-NB100-168-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/20/5207), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0014.jpg "Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/20/5207), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Staining of human cell line U-2 OS shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. Image collected and cropped by CiteAb from the following publication (//jcs.biologists.org/lookup/doi/10.1242/jcs.186338), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81445-img0013.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0008.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human bronchus shows moderate to strong positivity in cilia in respiratory epithelial cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0009.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human nasopharynx shows strong positivity in cilia in respiratory epithelial cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0010.jpg)
![Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human endometrium shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/CEP164-Antibody-Immunohistochemistry-Paraffin-NBP1-81445-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. (A–D) Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. (E) Quantification results of the frequency of ciliated HTR8 cells. ** p < 0.01, results are the mean ± SD from three independent experiments, more than 100 cells were counted in each individual group. DNA was stained with DAPI. Scale bar 10 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31640125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-31020241539453.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Super-resolution (3D-SIM) microscopic analysis of centriolar distal-end proteins. (a) RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Lower panels show fluorescence profile plots. Representative images of more than ten cells are presented in (a). ND: nascent daughter centriole, M: mother centriole. (b) The diameters of C2CD3, Talpid3, CEP120, & CEP164 protein spots (n = 30/each). Cumulative data from three independent experiments are shown. ***P < 0.001. (c,d) 3D-SIM analysis of the spatial localizations of C2CD3 (c), Talpid3 (d) & CEP120 (c,d) at the distal ends of centrioles. RPE1 cells were synchronized at G2 phase & immunostained with the indicated antibodies. Scale bar, 100 nm in (a,c,d). (e) Schematic illustration of the spatial localizations of C2CD3, Talpid3, CEP120, & CP110 at the distal ends of centrioles. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30988386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416113546.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416121961.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - CPAP-E1235V mutation impaired the recruitment of centriole elongation proteins onto the centrioles. Cells were synchronized in the early S phase by treatment with aphidicolin (2 μg/ml) for 24 h. They were then fixed immediately with methanol (S phase centrioles) or released into fresh culture medium without aphidicolin for another 16 h (G2 phase centrioles). CPAP-WT or mutant cells in the S phase or G2 phase were immunostained with antibodies against the following centriolar proteins. (A) STIL, n = 42 for CPAP-WT; n = 25 for CPAP-E1235V#1; n = 34 for CPAP-E1235V#2. (B) CEP120, n = 50 for CPAP-WT; n = 42 for CPAP-E1235V#1; n = 48 for CPAP-E1235V#2. (C) CENTROBIN, n = 40 for all groups. (D) CEP295, n = 40 for all groups. (E) POC5, n = 42 for CPAP-WT; n = 40 for CPAP-E1235V#1; n = 40 for CPAP-E1235V#2. (F) POC1B, n = 40 for all groups. (G) CEP164, n = 88 for CPAP-WT; n = 74 for CPAP-E1235V#1; n = 38 for CPAP-E1235V#2. All data are presented as mean ±SEM from a pool of n cells from three independent experiments. n.s: not significant; **p < 0.01; ***p < 0.001. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35309908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-31020241613623.jpg)
![Immunocytochemistry/ Immunofluorescence: CEP164 Antibody [NBP1-81445] - Depletion of RTTN produces shorter centrioles. a, b U2OS cells were treated with siControl or siRTTN as shown in a, & analyzed by confocal fluorescence microscopy using the indicated antibodies. cHistogram illustrating the distance between the CEP162-positive dots associated with a given pair of orthogonally oriented centrioles. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). d, f PLK4-myc doxycycline (Dox) inducible cells were treated with siControl or siRTTN as shown in a, & then analyzed by confocal fluorescence microscopy using the indicated antibodies d, or by electron microscopy f. e, gHistogram illustrating the length of procentrioles in PLK4-myc-inducible cells, as analyzed by confocal microscopy e or electron microscopy g. Error bars represent the mean ± s.d.; ***P < 0.001 (two-tailed t-test). The procentriole length in b/c was measured as the distance between two CEP162 dots as described by Azimzadeh et al.17 The procentriole length in d/e was measured as the distance between the fluorescent peak intensity of SAS-6 (red) & CEP162 (green) in siControl & siRTTN-treated cells. Scale bar, 5 μm in b & d; Scale bar, 200 nm in f Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00305-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81445_rabbit-polyclonal-cep164-antibody-310202416114711.jpg)
![Immunocytochemistry/Immunofluorescence: ALKBH5 Antibody [NBP2-78817] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ALKBH5 Antibody NBP2-78817 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78817-img0001.jpg "Immunocytochemistry/Immunofluorescence: ALKBH5 Antibody [NBP2-78817] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ALKBH5 Antibody NBP2-78817 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0008.jpg "Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.")
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Immunohistochemical analysis of human breast cancer biopsies. Immunohistochemistry was performed on breast cancer biopsies using ALKBH5 and HIF-1a antibodies. Representative negative and positive staining (biopsies from patients #2 and #3, respectively) are shown. Scale bar: 500 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0023.jpg)
![Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0007.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0015.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0018.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0019.jpg)
![Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Immunohistochemistry-Paraffin-NBP1-82188-img0021.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: ALKBH5 Antibody [NBP1-82188] - ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levels. MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALKBH5-Antibody-Western-Blot-NBP1-82188-img0022.jpg)
![Western Blot: ALKBH5 Antibody [NBP1-82188] - ZNF217 & ALKBH5 regulate NANOG & KLF4 expression via modulation of m6A levels. J. MCF-7 subclones exposed to 20% or 1% O2 for 48 h & immunoblot assays performed. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82188_rabbit-polyclonal-alkbh5-antibody-31020241534366.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Western-Blot-NB100-692-img0003.jpg "Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin fixed paraffin embedded human tonsil stained with MGMT antibody.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0002.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT MT 3.1 (Cat. #NB 100-692) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0001.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - MMR protein expression & hMutS alpha activity decreases while hprt mutation rates & MNNG resistance increases in MGMT & MMR negative subclones after repeated exposure to MNNG.A. MMR protein expression, each fluorescent protein band was measured against a loading control (p62) in the same lane by Alpha lnnotech Fluorochem HD2, histograms produced by Prism GraphPad software, error bars indicate SD. U251 not represented in histogram because hMSH6 & hMSH2 expression is completely absent (Figure 1). Asterisks (*) denote statistically significant differences at P < 0.05 between each subclone protein expression & HeLa MR for the designated MMR protein. Statistical significance determined by student t-test using Prism GraphPad software. Each experiment was performed a minimum of 3 times. B. hMutS alpha binding activity of equal nuclear protein concentration from nuclear extracts of each cell line by EMSA using [32P]-69mer oligomers with either G:T or 06meG:T located in the center. C. hprt mutation rates in two sequentially isolated HeLa MNNGR clones as compared to HeLa MR. D. Classic colony survival of HeLa MNNG4 & U251R subclones indicates significant resistance to 0.2 µM MNNG as compared to HeLa MR & U251 parental cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24019948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-692_mouse-monoclonal-mgmt-antibody-mt-3-1-31020241612636.jpg)
![Western Blot: BLAP75 Antibody [NB100-1720] - Whole cell lysate (50 ug) from U2OS, HeLa, MCF-7, andLNCaP cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-BLAP75 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BLAP75-Antibody-Western-Blot-NB100-1720-img0015.jpg "Western Blot: BLAP75 Antibody [NB100-1720] - Whole cell lysate (50 ug) from U2OS, HeLa, MCF-7, andLNCaP cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-BLAP75 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: BLAP75 Antibody [NB100-1720] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BLAP75 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BLAP75-Antibody-Immunohistochemistry-Paraffin-NB100-1720-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BLAP75 Antibody [NB100-1720] - Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BLAP75 antibody NB100-1720 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/BLAP75-Antibody-Western-Blot-NB100-1720-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: USP11 Antibody [NBP1-80640] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80640-img0005.jpg "Immunocytochemistry/Immunofluorescence: USP11 Antibody [NBP1-80640] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-USP11 antibody NBP1-80640 (A) shows similar protein distribution across tissues to independent antibody NBP1-80639 (B).](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding USP11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0013.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human kidney.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0014.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0015.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0016.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human cerebellum shows weak nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0017.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80640] - Staining of human cerebral cortex shows weak to moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80640-img0020.jpg)
![Western Blot: NOX1 Antibody [NBP1-69573] - NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenocarcinoma CaCO2 at 1:10.](http://images.novusbio.com/fullsize/NOX1-Antibody-Western-Blot-NBP1-69573-img0003.jpg "Western Blot: NOX1 Antibody [NBP1-69573] - NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenocarcinoma CaCO2 at 1:10.")
![Western Blot: NOX1 Antibody [NBP1-69573] - This Anti-NOX1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 0.5ug/ml.](http://images.novusbio.com/fullsize/NOX1-Antibody-Western-Blot-NBP1-69573-img0002.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0019.jpg "Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-USP11 antibody NBP1-80639 (A) shows similar protein distribution across tissues to independent antibody NBP1-80640 (B).](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding USP11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0008.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0012.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0015.jpg)
![Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-Paraffin-NBP1-80639-img0018.jpg)
![Immunohistochemistry: USP11 Antibody [NBP1-80639] - Staining of mouse caudate putamen shows positivity in blood vessels.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-NBP1-80639-img0006.jpg)
![Immunohistochemistry: USP11 Antibody [NBP1-80639] - Staining of mouse caudate putamen shows positivity in blood vessels.](http://images.novusbio.com/fullsize/USP11-Antibody-Immunohistochemistry-NBP1-80639-img0010.jpg)
![Western Blot: MPG Antibody [NBP1-82787] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/MPG-Antibody-Western-Blot-NBP1-82787-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: MPG Antibody [NBP1-82787] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MPG Antibody [NBP1-82787] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MPG-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82787-img0012.jpg)
![Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/MPG-Antibody-Immunohistochemistry-Paraffin-NBP1-82787-img0011.jpg)
![Western Blot: MGMT Antibody (4F9) [NBP2-45843] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT.](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Western-Blot-NBP2-45843-img0018.jpg "Western Blot: MGMT Antibody (4F9) [NBP2-45843] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT.")
![Immunohistochemistry: MGMT Antibody (OTI4F9) [NBP2-45843] - MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-OTI4F9-Immunohistochemistry-NBP2-45843-img0019.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0001.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0002.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0003.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0004.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0005.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0006.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0007.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0008.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0009.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0010.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0012.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0013.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0014.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0015.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0016.jpg)
![Immunohistochemistry: MGMT Antibody (4F9) [NBP2-45843] - Analysis of Human tonsil within the normal limits using MGMT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/MGMT-Antibody-4F9-Immunohistochemistry-NBP2-45843-img0017.jpg)